{"id":1099,"date":"2025-08-26T07:32:10","date_gmt":"2025-08-26T07:32:10","guid":{"rendered":"https:\/\/kromogen.com\/?page_id=1099"},"modified":"2025-08-26T07:32:10","modified_gmt":"2025-08-26T07:32:10","slug":"hla-tiplendirme","status":"publish","type":"page","link":"https:\/\/kromogen.com\/en\/hla-typing\/","title":{"rendered":"HLA Typing"},"content":{"rendered":"

NanoTYPE 24\/11 - High Resolution HLA Genotyping<\/strong>
\nThe Reliable Solution for Determining Transplantation Compliance<\/em><\/p><\/blockquote>\n

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  1. Product Description<\/strong><\/li>\n<\/ol>\n

    NanoTYPE 24\/11 is a CE approved in-vitro diagnostic (IVD) kit for high-resolution genotyping of HLA genes. It is used to assess recipient-donor compatibility in organ and stem cell transplants.<\/p>\n

      \n
    1. Targeted Genes<\/strong><\/li>\n<\/ol>\n

      Kit, in a single study Class I (HLA-A, HLA-B, HLA-C) and Class II (HLA-DRB1, HLA-DRB3\/4\/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, HLA-DPB1)<\/strong> totaling 11 HLA<\/strong> gene at the same time.<\/p>\n\n\n\n\n\n\n\n\n\n
      \n

      HLA Gene<\/em><\/strong><\/p>\n<\/td>\n

      		\n

      Coverage<\/em><\/strong><\/p>\n<\/td>\n<\/tr>\n

      \n

      HLA-A, B, C, DQA1, DQB1 and DPA1<\/p>\n<\/td>\n

      		All Gen<\/td>\n<\/tr>\n
      \n

      DRB1<\/p>\n<\/td>\n

      		\n

      Exons 2-4<\/p>\n<\/td>\n<\/tr>\n

      \n

      DRB3<\/p>\n<\/td>\n

      		\n

      Exons 2-4<\/p>\n<\/td>\n<\/tr>\n

      \n

      DRB4<\/p>\n<\/td>\n

      		\n

      Exons 2-6<\/p>\n<\/td>\n<\/tr>\n

      \n

      DRB5<\/p>\n<\/td>\n

      		\n

      Exons 2-6<\/p>\n<\/td>\n<\/tr>\n

      DPB1<\/td>\n\n

      Exons 2-5<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

       <\/p>\n

        \n
      1. Technology<\/strong><\/li>\n<\/ol>\n
          \n
        • High resolution<\/strong> - Compliant with EFI and ASHI standards<\/li>\n
        • Fast result<\/strong> - High quality data in a short time (results in 5 hours for 1 sample)<\/li>\n
        • Comprehensive analysis<\/strong> - Evaluation of 11 loci in a single study<\/li>\n
        • Flexible use<\/strong> - Single or multiple sample analysis as needed<\/li>\n
        • CE approved IVD product<\/strong> - Should only be used in competent molecular diagnostic laboratories by trained personnel<\/li>\n<\/ul>\n

           <\/p>\n

            \n
          1. Method Principle<\/strong><\/li>\n<\/ol>\n

            These kits amplify 11 different HLA loci simultaneously in a single tube using a single long-read multiplex PCR method.<\/p>\n

            After PCR, barcoding, pooling, adapter addition and library size selection, if necessary, are performed according to the number of samples.<\/p>\n

            The prepared library is then loaded into Oxford Nanopore Technologies sequencing devices. During sequencing, DNA fragments generate unique signals in the electric field as they pass through the nanopores. These signals are converted into DNA sequences in FASTQ format by base caller software.<\/p>\n

            Finally, the sequences are analyzed with NanoTYPER\u2122 software to obtain HLA genotyping results.<\/p>\n

            \"\"<\/p>\n

             <\/p>\n

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